EMSA-Related

  
        

  

More and more researchers are using non-radioactive  techniques for DNA/RNA or protein detection. Non-radioactive detection primarily is depended on application of chemiluminescence catalyzed by HRP, AKP or other enzymes. The development of enhanced chemiluminescence (ECL) increases greatly the sensitivity of detection, and the sensitivity of chemiluminescence is considerably higher than that using radioisotopes for detection of DNA (Southern blotting), RNA (Northern blotting) and proteins (Western Blotting). EMSA Products

For example, using chemiluminescence, obtaining images usually takes only a few minutes, whereas obtaining autoradiographs with radioisotope-labeled molecules takes 16-48 hours.

Advantages for ECL-based detection:

High Sensitivity

Safer than using radioisotopes

Easy to handle

Much shorter time to obtain results

Long life for Biotin- or fluorescein-probes

In preparation of DNA/RNA-based non-radioactive probes, Biotin, DIG and fluorescein are used most commonly. Among these labeling molecules, Biotin is most widely used for its chemical stability, high affinity, fast binding time, small molecular weight and compact structure. The major technology developed by Viagene Biotech is high sensitive non-radioactive detection based on HRP-induced chemiluminescence. We have provided services for our customers in life sciences with non-radioactive detection kits since 2006. We have hundreds of premade biotin-labeled probes for EMSA and have years of experience for custom-synthesizing non-radioactive probes using either biotin or fluorescein labeling.

 

Catalog# Products Description Pack Price($)
SIDET101 Coolshift-BId General ECL EMSA kit, DNA 100assays 277
SIDET102 Coolshift-BIr General ECL EMSA kit, RNA 100assays 399
SIDET201 Coolshift-IRd General IR EMSA kit, DNA 100assays 199
SIDET202 Coolshift-IRr General IR EMSA kit, RNA 100assays 298
BITFxxx detail Complete ECL EMSA kits 30/60assays 287/492
IRTFxxx detail Complete IR EMSA kits 30/60assays 287/492
IRTFxxx detail Gel-staining EMSA kits 50/100assays 129/198
SERV005a1 EMSA-PN Ctrl EMSA/positive+negatives 5 loads/ea. 95
SERV005a2 EMSA-PN Ctrl EMSA/positive+negatives 10 loads/ea. 155
SERV005b1 EMSA-P Ctrl EMSA/positive controls 5 loads/ea. 75
SERV005b2 EMSA-P Ctrl EMSA/positive controls 10 loads/ea. 119
SERV005c1 EMSA-N Ctrl EMSA/negative controls 5 loads/ea. 45
SERV005c2 EMSA-N Ctrl EMSA/negative controls 10 loads/ea. 59
TFRGT105a Poly[dI:dC] EMSA/non-specific inhibitor 50assays 64
TFRGT105b Poly[dI:dC] EMSA/non-specific inhibitor 100assys 99
TFRGT108a EMSA-Mmb EMSA/probe-membranes 5 sheets 55
TFRGT108b EMSA-Mmb EMSA/probe-membranes 10 sheets 99
TFRGT104a EMSA-RBu EMSA/reaction buffer 50assays 25
TFRGT104b EMSA-RBu EMSA/reaction buffer 100assays 35
TFRGT110a ESA-HRPb Streptavidin-HRP 50assays 65
TFRGT110b ESA-HRPb Streptavidin-HRP 100assys 99
TFRGT103a EMSA-BBuf ECL EMSA/blocking buf. 60ml/btl 45
TFRGT103b EMSA-WBuf ECL  EMSA/washing buf. 60ml/btl 59
TFRGT103c EMSA-EBuf ECL  EMSA/equilibration buf. 60ml/btl 21
TFxxxBP details Biotin-probes for EMSA 50assays 99
TFxxxBP details Biotin-probes for EMSA 100assys 159
TFxxxIR details IR Fluo-probes for EMSA 50assys 135
TFxxxIR details IR Fluo-probes for EMSA 100assys 199
TFxxxCP details Unlabeled competitive probes 6assays 45
TFxxxCP details Unlabeled competitive probes 12assays 65
TFxxxMP details Unlabeled mutant probes 6assays 59
TFxxxMP details Unlabeled mutant probes 12assays 89
SINP001a C-N extractn Cell Nuclear Extraction 50samples 99
SINP001b T-N extractn Tissue Nuclear Extraction 50samples 125
SINP013 EMSA-ProC Nucl. protein concentration 100mL/botl 85


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