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RNA interference (RNAi), is a technique in which exogenous,
double-stranded RNAs (dsRNAs) are introduced into cells to
destroy specifically targeted mRNA, thereby diminishing or
abolishing expression of the gene of interest. The technique
has proven effective in Drosophila, Caenorhabditis
elegans, plant and mammalian cells.
In the mammalian cell, siRNA which has more than 30bp can
induce an activation of interferon, which can suppress mRNA
translation non-specifically. Some 10-21 nucleotides (called
siRNAs) are carried into the cell, and they can destroy mRNA
specifically. Thus, RNAi is a gene-silencing technique used
to analyze the molecular and cell biologic effects of
knockdown of expression of specific genes. |
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For many applications, it is undesirable or inefficient to
incorporate exogenous naked siRNA molecules into cells.
Alternatively, a powerful way to express siRNA in cells is to
incorporate the desired siRNA sequence into a vector which
results in efficient transfection and transduction in the
desired cell type, using a U5 or H promoter that will drive
production of the siRNA in the cell. This is accomplished by
incorporating into the vector what is called a short hairpin RNA
(shRNA) sequence, which contains reverse complementary sequences
of the siRNA separated by a short non-complementary nucleotide
sequence, which will form annealed double-stranded siRNA with an
intervening shortsingle-stranded hairpin loop, which can be
processed by the endogenous DICER system to release siRNA
molecules intracellularly.
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A. shRNA Vectors
pU6Mega-si/zeo Vector
[Cat#:
RNIV001]
The pU6Mega-si/zeo Vector with U6 promoter contains fewer
than 2400bp. The operation is extremely simple. You can ligate
the vector, which has already been predigested and tested with
your shRNA insert, and get positive clones within one day. In
addition, more than one shRNA (multiple copies of the same shRNA,
multiple different shRNAs targeting the same mRNA, or shRNA
against multiple target mRNAs can be carried in a single vector.
this vector system is suitable for all prokaryotic and
eukaryotic cell systems.
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pH1Mega-si/zeo Vector [Cat#:
RNIV002]
The pH1Mega-si/zeo Vector with H1 promoter contains fewer
than 2400bp. The operation is extremely simple. You can ligate
the vector, which has already been predigested and tested with
your shRNA insert, and get positive clones within one day. In
addition, more than one shRNA (multiple copies of the same shRNA,
multiple different shRNAs targeting the same mRNA, or shRNA
against multiple target mRNAs can be carried in a single vector.
this vector system is suitable for all prokaryotic and
eukaryotic cell systems.
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pH1U6Mega-si/zeo Vector
[Cat#:
RNIV003]
The pH1U6Mega-si/zeo Vector with H1 and U6 promoters
contains fewer than 2400bp. The operation is extremely simple.
You can ligate the vector, which has already been predigested
and tested with your shRNA insert, and get positive clones
within one day. In addition, more than one shRNA (multiple
copies of the same shRNA, multiple different shRNAs targeting
the same mRNA, or shRNA against multiple target mRNAs can be
carried in a single vector. this vector system is suitable for
all prokaryotic and eukaryotic cell systems.
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pH7U6Mega-si/zeo Vector
[Cat#:RNIV004]
The pH7U6Mega-si/zeo Vector with H7SK and U6 promoters
contains fewer than 2400bp. The operation is extremely simple.
You can ligate the vector, which has already been predigested
and tested with your shRNA insert, and get positive clones
within one day. In addition, more than one shRNA (multiple
copies of the same shRNA, multiple different shRNAs targeting
the same mRNA, or shRNA against multiple target mRNAs can be
carried in a single vector. this vector system is suitable for
all prokaryotic and eukaryotic cell systems.
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pH7Mega-si/zeo Vector
[Cat#:RNIV005]
The pH7Mega-si/zeo Vector with H7SK promoter contains fewer
than 2400bp. The operation is extremely simple. You can
ligate the vector, which has already been predigested and
tested with your shRNA insert, and get positive clones
within one day. In addition, more than one shRNA (multiple
copeis of the same shRNA, multiple different shRNAs
targeting the same mRNA, or shRNA against multiple target
mRNAs can be carried in a single vector. this vector system
is suitable for all prokaryotic and eukaryotic cell systems.
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*shRNA/siRNA tools |
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B. Multiple-Copies
Expression |
Multiple shRNA kit [Cat#: RNIK001]
This is a kit to insert multiple shRNA s in PU6Mega-si/zeo
or pH1Mega-si/zeo to increase efficiency of knockdown of
expression of a single mRNA or to knock down expression of
multiple mRNAs at the same time. |
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